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b cell isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec b cell isolation kit
    B Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+b+cell+isolation+kit/B+Cell+Isolation+Kit%2C+mouse/pm42862270-50-12-16
    Average 96 stars, based on 1026 article reviews
    b cell isolation kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    In Vitro:

    Article Title: Tet2 deficiency promotes IgG1+ B‐cell expansion and differentiation blockade through deregulation of the Nfkbia –c‐Rel axis
    Article Snippet: .. In vitro ‐iGCB, iPC, and induced memory B cells (iMBC) were generated and defined according to established protocols and nomenclature., Splenic murine naïve B‐cells were isolated from 2‐ to 3‐month‐old mice using the mouse B‐cell isolation kit (CD43 (Ly‐48) MicroBeads; Miltenyi Biotec) according to the manufacturer's instructions (yield >90% CD19+ B220+). .. Naïve B‐cells were cultured in the presence of 40LB‐cells expressing CD40L and producing BAFF as described previously., Briefly, purified B‐cells (110 × 10 3 cells per well) were cultured in a 6‐well plate in the presence of a confluent layer of 40LB‐cells that had been irradiated with 80 Gy of γ‐ray. rmIL‐4 (1 ng/mL; Peprotech) was added to the primary culture for 4 days, and on Day 4, the cells were replated onto a new irradiated feeder layer and cultured with rmIL‐21 (10 ng/mL; Peprotech).

    Generated:

    Article Title: Tet2 deficiency promotes IgG1+ B‐cell expansion and differentiation blockade through deregulation of the Nfkbia –c‐Rel axis
    Article Snippet: .. In vitro ‐iGCB, iPC, and induced memory B cells (iMBC) were generated and defined according to established protocols and nomenclature., Splenic murine naïve B‐cells were isolated from 2‐ to 3‐month‐old mice using the mouse B‐cell isolation kit (CD43 (Ly‐48) MicroBeads; Miltenyi Biotec) according to the manufacturer's instructions (yield >90% CD19+ B220+). .. Naïve B‐cells were cultured in the presence of 40LB‐cells expressing CD40L and producing BAFF as described previously., Briefly, purified B‐cells (110 × 10 3 cells per well) were cultured in a 6‐well plate in the presence of a confluent layer of 40LB‐cells that had been irradiated with 80 Gy of γ‐ray. rmIL‐4 (1 ng/mL; Peprotech) was added to the primary culture for 4 days, and on Day 4, the cells were replated onto a new irradiated feeder layer and cultured with rmIL‐21 (10 ng/mL; Peprotech).

    Isolation:

    Article Title: Tet2 deficiency promotes IgG1+ B‐cell expansion and differentiation blockade through deregulation of the Nfkbia –c‐Rel axis
    Article Snippet: .. In vitro ‐iGCB, iPC, and induced memory B cells (iMBC) were generated and defined according to established protocols and nomenclature., Splenic murine naïve B‐cells were isolated from 2‐ to 3‐month‐old mice using the mouse B‐cell isolation kit (CD43 (Ly‐48) MicroBeads; Miltenyi Biotec) according to the manufacturer's instructions (yield >90% CD19+ B220+). .. Naïve B‐cells were cultured in the presence of 40LB‐cells expressing CD40L and producing BAFF as described previously., Briefly, purified B‐cells (110 × 10 3 cells per well) were cultured in a 6‐well plate in the presence of a confluent layer of 40LB‐cells that had been irradiated with 80 Gy of γ‐ray. rmIL‐4 (1 ng/mL; Peprotech) was added to the primary culture for 4 days, and on Day 4, the cells were replated onto a new irradiated feeder layer and cultured with rmIL‐21 (10 ng/mL; Peprotech).

    Article Title: T-cell-targeted fusogenic nanovesicles generate CAR-T cells in vivo for rheumatoid arthritis therapy.
    Article Snippet: All cells were analyzed using BD FACSCelesta and cell viability was consistently above 90% based on trypan blue exclusion. .. Isolation and Culture of Mouse B cells: B cells were carefully isolated from the spleens of C57BL/6 mice using the Mouse B Cell Isolation Kit (Miltenyi, Germany). ..

    Article Title: B cell-intrinsic IL-2 signaling regulates inflammation by promoting IL-10 expression in CD25 + age-associated B cells.
    Article Snippet: When required, after obtaining cell suspension, red blood cells were lysed with 1 mL of RBC lysis buffer (Thermo Fisher Scientific, 00-4333-57) for 2.5 minutes at 4◦C. .. Splenic B cells were isolated using Mouse B cell isolation kit (Miltenyi Biotec, 130-090-862). .. Splenic PDCA-1pos and PDCA-1neg B cells were isolated from isolated splenic B cells using anti-mPDCA-1 microbeads, Mouse (Miltenyi Biotec, 130-091-965).

    Article Title: Tet2 deficiency promotes IgG1+ B‐cell expansion and differentiation blockade through deregulation of the Nfkbia –c‐Rel axis
    Article Snippet: .. Naïve B‐cells were isolated from the spleens of 2‐ to 3‐month‐old mice using the mouse B‐cell isolation kit (CD43 (Ly‐48) MicroBeads; Miltenyi Biotec) with a yield exceeding 85% CD19+ B220+. ..

    Article Title: Tet2 deficiency promotes IgG1+ B‐cell expansion and differentiation blockade through deregulation of the Nfkbia –c‐Rel axis
    Article Snippet: .. At Day 0, splenic murine naïve B‐cells from a Tet2 ‐KO mouse were isolated from 3 to 4‐month‐old mice using the mouse B‐cell isolation kit (CD43 (Ly‐48) MicroBeads; Miltenyi Biotec) following the manufacturer's instructions (yield >90% CD19+ B220+). ..

    Article Title: Immune-induced TCR-like antibodies regulate specific T cell response in mice
    Article Snippet: .. In vivo assay, B cells were isolated from the spleen of eight to ten-week-old female B10.A(4 R) and B10.A(4 R)-MD4 mice using the mouse B cell isolation kit (130-090-862, Miltenyi Biotec). .. These B cells were co-cultured with 10ng/ml IL-4 (404-ML-010, R&D Systems), 5 μg/ml anti-CD40 antibody (102812, Biolegend), 5 μg/ml anti-IgM antibody (115-006-075, Jackson Immuno Research) with or without HEL protein for 18h.

    Article Title: T-cell-targeted fusogenic nanovesicles generate CAR-T cells in vivo for rheumatoid arthritis therapy
    Article Snippet: All cells were analyzed using BD FACSCelesta and cell viability was consistently above 90% based on trypan blue exclusion. .. B cells were carefully isolated from the spleens of C57BL/6 mice using the Mouse B Cell Isolation Kit (Miltenyi, Germany). ..

    Cell Isolation:

    Article Title: T-cell-targeted fusogenic nanovesicles generate CAR-T cells in vivo for rheumatoid arthritis therapy.
    Article Snippet: All cells were analyzed using BD FACSCelesta and cell viability was consistently above 90% based on trypan blue exclusion. .. Isolation and Culture of Mouse B cells: B cells were carefully isolated from the spleens of C57BL/6 mice using the Mouse B Cell Isolation Kit (Miltenyi, Germany). ..

    Article Title: B cell-intrinsic IL-2 signaling regulates inflammation by promoting IL-10 expression in CD25 + age-associated B cells.
    Article Snippet: When required, after obtaining cell suspension, red blood cells were lysed with 1 mL of RBC lysis buffer (Thermo Fisher Scientific, 00-4333-57) for 2.5 minutes at 4◦C. .. Splenic B cells were isolated using Mouse B cell isolation kit (Miltenyi Biotec, 130-090-862). .. Splenic PDCA-1pos and PDCA-1neg B cells were isolated from isolated splenic B cells using anti-mPDCA-1 microbeads, Mouse (Miltenyi Biotec, 130-091-965).

    Article Title: Immune-induced TCR-like antibodies regulate specific T cell response in mice
    Article Snippet: .. In vivo assay, B cells were isolated from the spleen of eight to ten-week-old female B10.A(4 R) and B10.A(4 R)-MD4 mice using the mouse B cell isolation kit (130-090-862, Miltenyi Biotec). .. These B cells were co-cultured with 10ng/ml IL-4 (404-ML-010, R&D Systems), 5 μg/ml anti-CD40 antibody (102812, Biolegend), 5 μg/ml anti-IgM antibody (115-006-075, Jackson Immuno Research) with or without HEL protein for 18h.

    Article Title: T-cell-targeted fusogenic nanovesicles generate CAR-T cells in vivo for rheumatoid arthritis therapy
    Article Snippet: All cells were analyzed using BD FACSCelesta and cell viability was consistently above 90% based on trypan blue exclusion. .. B cells were carefully isolated from the spleens of C57BL/6 mice using the Mouse B Cell Isolation Kit (Miltenyi, Germany). ..

    In Vivo:

    Article Title: Immune-induced TCR-like antibodies regulate specific T cell response in mice
    Article Snippet: .. In vivo assay, B cells were isolated from the spleen of eight to ten-week-old female B10.A(4 R) and B10.A(4 R)-MD4 mice using the mouse B cell isolation kit (130-090-862, Miltenyi Biotec). .. These B cells were co-cultured with 10ng/ml IL-4 (404-ML-010, R&D Systems), 5 μg/ml anti-CD40 antibody (102812, Biolegend), 5 μg/ml anti-IgM antibody (115-006-075, Jackson Immuno Research) with or without HEL protein for 18h.



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